DIFFERENTIATION OF ADAPTATIONS OF ATHLETES IN ERGOESPIROMETRIC EXERCISE TEST BY INFRARED SPECTROSCOPY

Abstract

Twelve athletes of Athletism team of UNISC were submitted to Bruce protocol on the treadmill (ergometer) connected to the spirometry system (respiratory gas analysis) being evaluated to the hearth rate, maximal oxygen uptake (VO2max), maximal carbon dioxide production (CO2max) and respiratory quocient (QR) at rest (pretest) and every 20 seconds until three minutes after the end of test (exhaustion or end of protocol). Blood pression was verified at rest, at transition between two states and after recovery. Blood collection by braquial vein puncture was carried at pretest and 10 min after the end of protocol (postest) and the blood samples were analysed by DRIFTS by 20 areas of bands. Significant differences (p < 0.05) were observed between sexes for 16 of 18 spectral bands. Differences in the postest stage were only observed with bands ?C=C-H, ?asC-H(CH3), ?asC-H(CH2), ?sC-H(CH3), ?sC-H(CH2), ?N-H(amide II) and ?sC=O(CO2-). The best univariate correlations with VO2max were observed with band C-O(1140-1120 cm-1) for male athletes (r = 0.960) and ?C=C-H(1140-1120 cm-1) for female athletes (r = 0.893). It can be conclude that whole blood FT-IR spectroscopy is an auxiliary tool to assess differences between male and female athletes in adaptations to ergospirometric exercise test.